pubmedtime外泌体与脑出血

Exosome-DerivedMiRNAsasBiomarkersoftheDevelopmentandProgressionofIntracranialAneurysms.

LiaoB1,ZhouMX1,ZhouFK1,LuoXM1,ZhongSX1,ZhouYF1,QinYS1,LiPP1,QinC1.

Authorinformation

1

DepartmentofNeurology,theFirstAffiliatedHospital,GuangxiMedicalUniversity.

AbstractAIM:

Exosome-derivedmicroRNAs(miRNAs)arepotentialdiagnosticbiomarkers.However,littleisknownabouttheireffectivenessasdiagnosticbiomarkersofintracranialaneurysms(IAs).ThisstudyaimedtoexploremiRNAlevelsinplasmaexosomesofpatientswithIAtoidentifypotentialbiomarkersthatpredictthedevelopmentandprogressofIA.

外来体来源的microRNA（miRNA）是潜在的诊断生物标志物。然而，关于它们作为颅内动脉瘤（IAs）的诊断生物标志物的有效性知之甚少。这项研究旨在探讨IA患者血浆外泌体中的miRNA水平，以鉴定预测IA发展和进展的潜在生物标志物。

METHODS:

Atotalof69patientswithIAand30healthycontrols(HC)wererecruited,amongwhom30hadunrupturedIA(UA),and39hadrupturedIA(RA).ThemiRNAexpressionprofilesofplasmaexosomesin12IApatients(4UAand8RA)and4HCweredeterminedusingnext-generationsequencing.Inaddition,significantlydifferentiallyexpressedmiRNAswerefurtheranalyzedbyQuantitativeReal-TimePCR(qRT-PCR)inavalidationcohortof99subjects.

总共招募了69位IA患者和30位健康对照（HC），其中30位未破裂的IA（UA）和39位破裂的IA（RA）。使用下一代测序技术确定了12名IA患者（4UA和8RA）和4HC中血浆外泌体的miRNA表达谱。此外，在99名受试者的验证队列中，通过定量实时PCR（qRT-PCR）对显着差异表达的miRNA进行了进一步分析。

RESULTS:

Fromthesequencinganalysis,miRNAswereidentifiedtobedifferently(p＜0.05)expressed.Ofthese,9miRNAswereup-regulated,and20weredown-regulatedinpatientswithUA

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